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| Reduced p21(WAF1/CIP1) expression and p53 mutation in hepatocellular carcinomas |
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| Author(s): Hui AM, Kanai Y, Sakamoto M, Tsuda H, Hirohashi S |
| Source: HEPATOLOGY Volume: 25 Issue: 3 Pages: 575-579 Published: MAR 1997 |
| Times Cited: 61 References: 41 |
| Abstract: p21(WAF1/CIP1) is a universal cyclin-dependent kinase (cdk) inhibitor, the expression of which is regulated by p53-dependent and p53-independent pathways. We examined p21(WAF1/CIP1) expression in and p53 status of 21 primary hepatocellular carcinomas (HCCs) by reverse-transcriptase polymerase chain reaction (RT-PCR) and by PCR single-strand conformation polymorphism (PCR-SSCP) analysis. p21(WAF1/CIP1) messenger RNA expression was reduced markedly in 8 of 21 HCCs (38.1%) and 5 of these 8 HCCs bore p53 mutations. The relative p21(WAF1/CIP1) messenger RNA expression value of HCCs with p53 mutations (.73 +/- .13 U, n = 6) was significantly lower than that of HCCs with wild-type p53 (1.00 +/- 0.21 U, n = 14; P < .01). The p21(WAF1/CIP1) expression levels in cancerous tissues (.73 +/- .13 U) were significantly reduced in comparison with those in noncancerous tissues (.97 +/- .13 U) (P < .01) in the 6 cases with p53 mutations. These data indicate that p21(WAF1/CIP1) expression in HCCs is predominantly regulated by dependence on p53 and that reduced p21(WAF1/CIP1) expression may participate in hepatocarcinogenesis. |
| Document Type: Article |
| Language: English |
Addresses:
1. NATL CANC CTR, RES INST, DIV PATHOL, CHUO KU, TOKYO 104, JAPAN |
| Publisher: W B SAUNDERS CO, INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 |
| Subject Category: Gastroenterology & Hepatology |
| IDS Number: WL830 |
| ISSN: 0270-9139 |
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