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| Extension of the replicative life span of human diploid fibroblasts by inhibition of the p33(ING1) candidate tumor suppressor |
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| Author(s): Garkavtsev I, Riabowol K |
| Source: MOLECULAR AND CELLULAR BIOLOGY Volume: 17 Issue: 4 Pages: 2014-2019 Published: APR 1997 |
| Times Cited: 107 References: 33 |
| Abstract: Previous studies suggest that tumor suppressors may play significant roles in blocking the growth of cells during cellular senescence, We therefore studied the potential involvement of a novel growth inhibitor and candidate tumor suppressor gene called ING1, which we have cloned recently (I. Garkavtsev, A. Kazarov A. Gudkov, and K. Riabowol, Nat, Genet, 14:415-420, 1996), in the process of cellular senescence, Our results show that the RNA and protein levels of ING1 were 8- to 10-fold higher in senescent cells than in young, proliferation-competent human diploid fibroblasts. Expression of the nuclear p33(ING1) during the cell cycle, reaching maximal levels during DNA synthesis, Chronic expression of antisense ING1 RNA reproducibly resulted in extension of the proliferative life span of normal human fibroblasts by approximately seven population doublings. |
| Document Type: Article |
| Language: English |
| Reprint Address: Garkavtsev, I (reprint author), UNIV CALGARY, DEPT MED BIOCHEM, CALGARY, AB T2N 4N1 CANADA |
Addresses:
1. UNIV CALGARY, DEPT ONCOL, CALGARY, AB T2N 4N1 CANADA
2. UNIV CALGARY, SO ALBERTA CANC RES CTR, CALGARY, AB T2N 4N1 CANADA |
| Publisher: AMER SOC MICROBIOLOGY, 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: WN906 |
| ISSN: 0270-7306 |
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