| | |  | | | | Record from Web of Science® | | | | | | |
| Prognostic impact of proliferation associated factors MIBI (Ki-67) and S-phase in node-negative breast cancer |
|
|
| Author(s): Dettmar P, Harbeck N, Thomssen C, Pache L, Ziffer P, Fizi K, Janicke F, Nathrath W, Schmitt M, Graeff H, Hofler H |
| Source: BRITISH JOURNAL OF CANCER Volume: 75 Issue: 10 Pages: 1525-1533 Published: MAY 1997 |
| Times Cited: 58 References: 42 |
| Abstract: MIB1 proliferation rate (MIB1-PR) and total S-phase fraction (SPF) were retrospectively determined in formalin-fixed, paraffin-embedded sections of 90 primary node-negative breast carcinomas. None of the patients had received adjuvant systemic therapy. Median follow-up in patients still alive at the time of analysis was 37.5 months (16-72 months). Immunostaining of Ki-67 antigen was pet-formed using the monoclonal antibody MIB1 and the APAAP technique. An adjacent 50-mu m paraffin section was used for flow cytometric S-phase determination. Results were compared to established clinicopathological prognostic factors. MIBI-PR was significantly correlated to grading (P = 0.018); SPF was significantly correlated with tumour size (P = 0.041) and inversely with steroid hormone receptor status (P = 0.03). A significant correlation between MIBI-PR and SPF was found in aneuploid (P = 0.025) but not in diploid tumours (P = 0.164). In univariate analysis, both MIB1-PR (optimized cut-off of 25%) and SPF (optimized cut-off of 8%) were significant prognostic factors for disease-free survival (DFS) (MIB1-PR, P = 0.0224; SPF, P = 0.0028). in multivariate analysis, however, only SPF remained significant; it was the strongest prognostic factor for DFS (P = 0.0073), stronger than MIB1-PR or established clinicopathological prognostic factors. We thus conclude that MIBI-PR and SPF provide additional prognostic information in node-negative breast cancer. However, in our study, flow cytometrically determined SPF had the greater prognostic impact. |
| Document Type: Article |
| Language: English |
Addresses:
1. TECH UNIV MUNICH, INST ALLGEMEINE PATHOL & PATHOL ANAT, D-81675 MUNICH, GERMANY
2. TECH UNIV MUNICH, FRAUENKLIN & POLIKLIN, D-81675 MUNICH, GERMANY |
| Publisher: CHURCHILL LIVINGSTONE, JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND |
| Subject Category: Oncology |
| IDS Number: WZ284 |
| ISSN: 0007-0920 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |