| | |  | | | | Record from Web of Science® | | | | | | |
| Positional cloning of the mouse circadian Clock gene |
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| Author(s): King DP, Zhao YL, Sangoram AM, Wilsbacher LD, Tanaka M, Antoch MP, Steeves TDL, Vitaterna MH, Kornhauser JM, Lowrey PL, Turek FW, Takahashi JS |
| Source: CELL Volume: 89 Issue: 4 Pages: 641-653 Published: MAY 16 1997 |
| Times Cited: 611 References: 74 |
| Abstract: We used positional cloning to identify the circadian Clock gene in mice. Clock is a large transcription unit with 24 exons spanning similar to 100,000 bp of DNA from which transcript classes of 7.5 and similar to 10 kb arise. Clock encodes a novel member of the bHLH-PAS family of transcription factors. In the Clock mutant allele, an A-->T nucleotide transversion in a splice donor site causes exon skipping and deletion of 51 amino acids in the CLOCK protein. Clock is a unique gene with known circadian function and with features predicting DNA binding, protein dimerization, and activation domains. CLOCK represents the second example of a PAS domain-containing clock protein (besides Drosophila PERIOD), which suggests that this motif may define an evolutionarily conserved feature of the circadian clock mechanism. |
| Document Type: Article |
| Language: English |
Addresses:
1. NORTHWESTERN UNIV, DEPT NEUROBIOL & PHYSIOL, NATL SCI FDN CTR BIOL TIMING, EVANSTON, IL 60208 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: WZ323 |
| ISSN: 0092-8674 |
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