| | |  | | | | Record from Web of Science® | | | | | | |
| Detection of coxsackie B virus RNA sequences in whole blood samples from adult patients at the onset of type I diabetes mellitus |
|
|
| Author(s): Andreoletti L, Hober D, HoberVandenberghe C, Belaich S, Vantyghem MC, Lefebvre J, Wattre P |
| Source: JOURNAL OF MEDICAL VIROLOGY Volume: 52 Issue: 2 Pages: 121-127 Published: JUN 1997 |
| Times Cited: 76 References: 26 |
| Abstract: Enteroviruses may be linked to insulin-dependent diabetes mellitus (IDDM). The prevalence of enteroviral (EV) infection at onset of adult IDDM was investigated by detection of specific EV sequences in peripheral blood using a reverse transcription and a seminested polymerase chain reaction (seminested RT-PCR). EDTA-treated whole blood samples taken from 12 newly diagnosed IDDM patients with ketosis or ketoacidosis were examined. The comparison groups were 12 adult patients suffering from metabolic decompensation in the course of IDDM, 12 adult patients with decompensated non-IDDM, and 15 healthy adults without any presumed EV infection or metabolic disease. EV genome was detected in five of 12 (42%) newly diagnosed IDDM patients and in one of 12 (8%) patients in the course of IDDM. By contrast, none of the 12 non-IDDM patients and none of the 15 healthy adults had EV sequences in whole blood. Subsequent sequencing of the EV PCR products from the six positive patients showed a significant homology with Coxsackie B3 or B4 viruses, and some common patterns were observed among the sequences. The present study demonstrates that Coxsackie B virus RNA sequences can be detected in peripheral blood from patients at the onset or in the course of IDDM and provides evidence for a role for enteroviruses in adult type I diabetes. (C) 1997 Wiley-Liss, Inc. |
| Document Type: Article |
| Language: English |
Addresses:
1. CTR HOSP REG & UNIV LILLE, VIROL LAB, F-59037 LILLE, FRANCE
2. CTR HOSP REG & UNIV LILLE, DEPT ENDOCRINOL & MALAD METAB, F-59037 LILLE, FRANCE |
| Publisher: WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 |
| Subject Category: Virology |
| IDS Number: XB771 |
| ISSN: 0146-6615 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |