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| Human CD4(+) cells transfected with IL-16 cDNA are resistant to HIV-1 infection: Inhibition of mRNA expression |
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| Author(s): Zhou P, Goldstein S, Devadas K, Tewari D, Notkins AL |
| Source: NATURE MEDICINE Volume: 3 Issue: 6 Pages: 659-664 Published: JUN 1997 |
| Times Cited: 84 References: 31 |
| Abstract: Interleukin-16 (IL-16) is secreted by activated CD8+ T lymphocytes and acts on CDC T lymphocytes, monocytes and eosinophils. Recently, the C-terminal 130-amino acid portion of IL-16 was shown to suppress HIV-1 replication in vitro. To explore the potential of human IL-16 for gene therapy, this portion was transfected into HIV-1-susceptible CD4(+) jurkat cells by means of a mammalian expression vector. The stable transfectants synthesized and secreted IL-16 protein. The expression of IL-16 did not alter growth rate and CD4 expression; however, HIV replication was inhibited by as much as 99%. Furthermore, during the initial phase of the infection, equal amounts of HIV-1 proviral DNA were found in cells transfected with IL-16 and with vector alone. In contrast, the 2-kilobase HIV-1 transcripts were markedly reduced and the 4-kb and 9-kb transcripts were undetectable in the cells transfected with IL-16. These findings indicate that IL-16-mediated inhibition of HIV-1 is not at the level of viral entry or reverse transcription, but at messenger RNA expression. |
| Document Type: Article |
| Language: English |
| Reprint Address: Zhou, P (reprint author), NIDR, BRANCH ORAL INFECT & IMMUN, NIH, BETHESDA, MD 20892 USA |
Addresses:
1. NIAID, IMMUNODEFICIENCY VIRUS SECT, INFECT DIS LAB, NIH, ROCKVILLE, MD 20852 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental |
| IDS Number: XB974 |
| ISSN: 1078-8956 |
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