| | |  | | | | Record from Web of Science® | | | | | | |
| HSV-TK gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia |
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| Author(s): Bonini C, Ferrari G, Verzeletti S, Servida P, Zappone E, Ruggieri L, Ponzoni M, Rossini S, Mavilio F, Traversari C, Bordignon C |
| Source: SCIENCE Volume: 276 Issue: 5319 Pages: 1719-1724 Published: JUN 13 1997 |
| Times Cited: 615 References: 41 |
| Abstract: In allogeneic bone marrow transplantation (allo-BMT), donor lymphocytes play a central therapeutic role in both graft-versus-leukemia (GvL) and immune reconstitution. However, their use is limited by the risk of severe graft-versus-host disease (GVHD). Eight patients who relapsed or developed Epstein-Barr virus-induced lymphoma after T cell-depleted BMT were then treated with donor lymphocytes transduced with the herpes simplex virus thymidine kinase (HSV-TK) suicide gene. The transduced lymphocytes survived for up to 12 months, resulting in antitumor activity in five patients. Three patients developed GVHD, which could be effectively controlled by ganciclovir-induced elimination of the transduced cells. These data show that genetic manipulation of donor lymphocytes may increase the efficacy and safety of allo-BMT and expand its application to a larger number of patients. |
| Document Type: Article |
| Language: English |
Addresses:
1. IST SCI SAN RAFFAELE, TELETHON INST GENE THERAPY, I-20132 MILAN, ITALY
2. MOLMED SPA, I-20132 MILAN, ITALY
3. IST SCI SAN RAFFAELE, DEPT PATHOL, I-20132 MILAN, ITALY |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: XD947 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |