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| Interleukin-6 functions as an autocrine growth factor in human bladder carcinoma cell lines in vitro |
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| Author(s): Okamoto M, Hattori K, Oyasu R |
| Source: INTERNATIONAL JOURNAL OF CANCER Volume: 72 Issue: 1 Pages: 149-154 Published: JUL 3 1997 |
| Times Cited: 37 References: 21 |
| Abstract: Interleukin (IL)-6 is reported to function as a growth factor for renal and prostatic carcinomas. We conducted the present study to define the role of IL-6 in the growth of normal and neoplastic urothelial cells, Human bladder carcinoma cell lines (253J, RT4 and T24) and primary cultured human urothelial cells derived from normal ureters were used, Recombinant human IL-6 stimulated the growth of bladder carcinoma cell lines far better than that of normal urothelial cells (p < 0.001). All carcinoma cell lines tested produced and released IL-6, whereas normal urothelial cells did so only at marginal levels. Furthermore, treatment with lipopolysaccharide derived from Escherichia coli, tumor necrosis factor-alpha or IL-1 increased IL-6 secretion by bladder carcinoma cell lines but not by normal urothelial cells. Growth of bladder carcinoma cells was significantly inhibited by anti-IL-6 neutralizing antibody or the anti-sense oligonucleotide for IL-6 cDNA. We conclude that IL-6 functions as an autocrine growth factor for bladder carcinoma cells but not for normal urothelial cells and that it may be a factor accounting for the marked enhancement of inflammation-associated bladder carcinogenesis and tumor growth. (C) 1997 Wiley-Liss, Inc. |
| Document Type: Article |
| Language: English |
Addresses:
1. NORTHWESTERN UNIV, DEPT PATHOL, SCH MED, CHICAGO, IL 60611 USA |
| Publisher: WILEY-LISS, DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 |
| Subject Category: Oncology |
| IDS Number: XG592 |
| ISSN: 0020-7136 |
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