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| Immune cell-derived beta-endorphin - Production, release, and control of inflammatory pain in rats |
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| Author(s): Cabot PJ, Carter L, Gaiddon C, Zhang Q, Schafer M, Loeffler JP, Stein C |
| Source: JOURNAL OF CLINICAL INVESTIGATION Volume: 100 Issue: 1 Pages: 142-148 Published: JUL 1 1997 |
| Times Cited: 149 References: 25 |
| Abstract: Localized inflammation of a rat's hindpaw elicits an accumulation of beta-endorphin-(END) containing immune cells, We investigated the production, release, and antinociceptive effects of lymphocyte-derived END in relation to cell trafficking, In normal animals, END and proopiomelanocortin mRNA were less abundant in circulating lymphocytes than in those residing in lymph nodes (LN), suggesting that a finite cell population produces END and homes to LN, Inflammation increased proopiomelanocortin mRNA in cells from noninflamed and inflamed LN, However, END content was increased only in inflamed paw tissue and noninflamed LN-immune cells, Accordingly, corticotropin-releasing factor and IL-1 beta released significantly more END from noninflamed than from inflamed LN-immune cells, This secretion was receptor specific, calcium dependent, and mimicked by potassium, consistent with vesicular release, Finally, both agents, injected into the inflamed paw, induced analgesia which was blocked by the co-administration of antiserum against END, Together, these findings suggest that END-producing lymphocytes home to inflamed tissue where they secrete END to reduce pain, afterwards they migrate to the regional LN, depleted of the peptide, Consistent with this notion, immunofluorescence studies of cell suspensions revealed that END is contained predominantly within memory-type T cells, Thus, the immune system is important for the control of inflammatory pain, This has implications for the understanding of pain in immunosuppressed conditions like cancer or AIDS. |
| Document Type: Article |
| Language: English |
| Reprint Address: Cabot, PJ (reprint author), NIDA, ADDICT RES CTR,BEHAV PHARMACOL & GENET SECT, INTRAMURAL RES PROGRAM,NIH, POB 5180, BALTIMORE, MD 21224 USA |
Addresses:
1. JOHNS HOPKINS UNIV HOSP, DEPT ANESTHESIOL & CRIT CARE MED, BALTIMORE, MD 21287 USA
2. UNIV STRASBOURG 1, LAB PHYSIOL GEN, F-67084 STRASBOURG, FRANCE |
| Publisher: ROCKEFELLER UNIV PRESS, 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 |
| Subject Category: Medicine, Research & Experimental |
| IDS Number: XK426 |
| ISSN: 0021-9738 |
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