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| A- and B-type lamins are differentially expressed in normal human tissues |
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| Author(s): Broers JLV, Machiels BM, Kuijpers HJH, Smedts F, vandenKieboom R, Raymond Y, Ramaekers FCS |
| Source: HISTOCHEMISTRY AND CELL BIOLOGY Volume: 107 Issue: 6 Pages: 505-517 Published: JUN 1997 |
| Times Cited: 74 References: 31 |
| Abstract: A selection of normal human tissues was investigated for the presence of lamins B1, B2, and A-type lamins, using a panel of antibodies specific for the individual lamin subtypes. By use of immunoprecipitation and two-dimensional immunoblotting techniques we demonstrated that these antibodies do not cross-react with other lamin subtypes and that a range of different phosphorylation isoforms is recognized by each antibody. The lamin B2 antibodies appeared to decorate the nuclear lamina in all tissues examined, except hepatocytes, in which very little lamin B2 expression was observed. In contrast to previous studies, which suggested the ubiquitous expression of lamin B1 in mammalian tissues, we show that lamin B1 is not as universally distributed throughout normal human tissues as was to be expected from previous studies. Muscle and connective tissues are negative, while in epithelial cells lamin B1 seemed to be preferentially detected in proliferating cells. These results correspond well with those obtained for lamin B1 in chicken tissues. The expression of A-type lamins is most prominent in well-differentiated epithelial cells. Relatively undifferentiated and proliferating cells in epithelia showed a clearly reduced expression of A-type lamins. Furthermore, most cells of neuroendocrine origin as well as most hematopoietic cells were negative for A-type lamin antibodies. |
| Document Type: Article |
| Language: English |
| Reprint Address: Broers, JLV (reprint author), UNIV MAASTRICHT, DEPT MOL GENET & CELL BIOL, POB 616, NL-6200 MD MAASTRICHT, NETHERLANDS |
Addresses:
1. CANISIUS WILHELMINA HOSP, DEPT PATHOL, NIJMEGEN, NETHERLANDS
2. HOP NOTRE DAME DE BON SECOURS, CTR RECH LC SIMARD, MONTREAL, PQ H2L 4K8 CANADA |
| Publisher: SPRINGER VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 |
| Subject Category: Cell Biology; Microscopy |
| IDS Number: XK998 |
| ISSN: 0301-5564 |
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