| Bcl-2 and the outer mitochondrial membrane in the inactivation of cytochrome c during fas-mediated apoptosis |
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| Author(s): Adachi S, Cross AR, Babior BM, Gottlieb RA |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 272 Issue: 35 Pages: 21878-21882 Published: AUG 29 1997 |
| Times Cited: 142 References: 21 |
| Abstract: Fas-driven apoptosis in Jurkat cells results in the inactivation of cytochrome c with cessation of oxygen consumption, Overexpression of Bcl-2 was found to protect against acidification and apoptosis mediated by Fas ligation in these cells. Bcl-2 is present in the outer mitochondrial membrane, but the molecular mechanism by which it protects cells is unknown. Because Bcl-2 projects into the mitochondrial intermembrane space and cytochrome c is located in the intermembrane space, we considered the possibility that Bcl-2 might protect cytochrome c from inactivation during Fas-mediated apoptosis. The present study shows that 1) in Jurkat cells, cytochrome c inactivation during Fas-driven apoptosis requires the permeabilization of the outer mitochondrial membrane; and 2) the post-mitochondrial fraction from CEM cells that overexpress Bcl-2 both prevents and reverses cytochrome c inactivation. |
| Document Type: Article |
| Language: English |
Addresses:
1. Scripps Res Inst, DEPT MOL & EXPT MED, LA JOLLA, CA 92037 USA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: XT850 |
| ISSN: 0021-9258 |