| | |  | | | | Record from Web of Science® | | | | | | |
| Fluorescence-based isolation of bacterial genes expressed within host cells |
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| Author(s): Valdivia RH, Falkow S |
| Source: SCIENCE Volume: 277 Issue: 5334 Pages: 2007-2011 Published: SEP 26 1997 |
| Times Cited: 346 References: 33 |
| Abstract: A selection strategy was devised to identify bacterial genes preferentially expressed when a bacterium associates with its host cell. Fourteen Salmonella typhimurium genes, which were under the control of at least four independent regulatory circuits, were identified to be selectively induced in host macrophages. Four genes encode virulence factors, including a component of a type III secretory apparatus. This selection methodology should be generally applicable to the identification of genes from pathogenic organisms that are induced upon association with host cells or tissues. |
| Document Type: Article |
| Language: English |
| Reprint Address: Valdivia, RH (reprint author), STANFORD UNIV, SCH MED, DEPT IMMUNOL & MICROBIOL, STANFORD, CA 94305 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: XX849 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |