| Exploring the metabolic and genetic control of gene expression on a genomic scale |
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| Author(s): DeRisi JL, Iyer VR, Brown PO |
| Source: SCIENCE Volume: 278 Issue: 5338 Pages: 680-686 Published: OCT 24 1997 |
| Times Cited: 2,590 References: 41 |
| Abstract: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration. The expression profiles observed for genes with known metabolic functions pointed to features of the metabolic reprogramming that occur during the diauxic shift, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions. The same DNA microarrays were also used to identify genes whose expression was affected by deletion of the transcriptional co-repressor TUP1 or overexpression of the transcriptional activator YAP1. These results demonstrate the feasibility and utility of this approach to genomewide exploration of gene expression patterns. |
| Document Type: Article |
| Language: English |
Addresses:
1. STANFORD UNIV, HOWARD HUGHES MED INST, SCH MED, DEPT BIOCHEM, STANFORD, CA 94305 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: YC323 |
| ISSN: 0036-8075 |