| | |  | | | | Record from Web of Science® | | | | | | |
| Cooking with calcium: The recipes for composing global signals from elementary events |
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| Author(s): Bootman MD, Berridge MJ, Lipp P |
| Source: CELL Volume: 91 Issue: 3 Pages: 367-373 Published: OCT 31 1997 |
| Times Cited: 223 References: 28 |
| Abstract: Recent studies have suggested that global intracellular Ca2+ signals arise from the summation and coordination of subcellular elementary release events (e.g., ''Ca2+ puffs''), although the modes of recruitment of such signals are unknown. In order to understand how cells utilize elementary Ca2+ release events, we imaged Ca2+ transients evoked through the phosphoinositide pathway in HeLa cells using confocal microscopy. During the pacemaker phase leading to the global Ca2+ signal, elementary Ca2+ release events were recruited in (1) frequency, (2) amplitude, and (3) spatial domains. Since each digital elementary event contributes to a small change of the analog cytosolic Ca2+ concentration, the net effect of the advancement in the three domains is to drive the ambient Ca2+ concentration toward a threshold where the signal becomes regenerative, resulting in a global Ca2+ wave. |
| Document Type: Article |
| Language: English |
Addresses:
1. BABRAHAM INST, MOL SIGNALLING LAB, CAMBRIDGE CB2 4AT, ENGLAND
2. UNIV CAMBRIDGE, DEPT ZOOL, CAMBRIDGE CB2 3EJ, ENGLAND |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: YD941 |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |