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| Tumor cell invasion through Matrigel is regulated by activated matrix metalloproteinase-2 |
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| Author(s): Deryugina EI, Luo GX, Reisfeld RA, Bourdon MA, Strongin A |
| Source: ANTICANCER RESEARCH Volume: 17 Issue: 5A Pages: 3201-3210 Published: SEP-OCT 1997 |
| Times Cited: 75 References: 43 |
| Abstract: We tested the hypothesis that there is a correlation between tumor cell efficiency in activation of matrix metalloproteinase-2 (MMP-2) and invasion through basement membrane-like Matrigel barriers. To generate cells capable of MMP-2 activation we stably transfected three human tumor cell fines, HT-1080 fibrosarcoma, MCF7 breast carcinoma, and U251.3 glioma with cDNA encoding the full length human membrane-type matrix metalloproteinase-1. Our results show a bimodal correlation between the extent of MMP-2 activation and Matrigel invasion by tumor cells. Cell transfectants characterized by a partial activation of MMP-2 were the most invasive while those with an extensive conversion of MMP-2 proenzyme into enzymatically active forms were the least efficient in invading Matrigel. Modulation of MMP-2 activation by exogenous TIMP-2 reverted the rare of Matrigel invasion by cell transfectants to control levels. We conclude that the regulation of activated MMP-2 in the tumor cells, microenvironment may be critical in facilitating tumor cell invasiveness. |
| Document Type: Article |
| Language: English |
Addresses:
1. LA JOLLA INST EXPT MED, LA JOLLA, CA 92037 USA
2. SCRIPPS RES INST, LA JOLLA, CA 92037 USA |
| Publisher: INT INST ANTICANCER RESEARCH, EDITORIAL OFFICE 1ST KM KAPANDNTIOU-KALAMOU RD KAPANDRITI, POB 22, ATHENS 19014, GREECE |
| Subject Category: Oncology |
| IDS Number: YK657 |
| ISSN: 0250-7005 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |