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| The Drosophila MSL complex acetylates histone h4 at lysine 16, a chromatin modification linked to dosage compensation |
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| Author(s): Smith ER, Pannuti A, Gu WG, Steurnagel A, Cook RG, Allis CD, Lucchesi JC |
| Source: MOLECULAR AND CELLULAR BIOLOGY Volume: 20 Issue: 1 Pages: 312-318 Published: JAN 2000 |
| Times Cited: 149 References: 46 |
| Abstract: In Drosophila, dosage compensation-the equalization of most X-linked gene products in males and females-is achieved by a twofold enhancement of the level of transcription of the X chromosome in males relative to each X chromosome in females. A complex consisting of at least five gene products preferentially binds the X chromosome at numerous sites in males and results in a significant increase in the presence of a specific histone isoform, histone 4 acetylated at lysine 16. Recently, RNA transcripts (roX1 and roX2) encoded by two different genes have also been found associated with the X chromosome in males. We have partially purified a complex containing MSL1, -2, and -3, MOF, MLE, and roX2 RNA and demonstrated that it exclusively acetylates H4 at lysine 16 on nucleosomal substrates. These results demonstrate that the MSL complex is responsible for the specific chromatin modification characteristic of the X chromosome in Drosophila males. |
| Document Type: Article |
| Language: English |
| Reprint Address: Lucchesi, JC (reprint author), Emory Univ, Dept Biol, 1510 Clifton Rd, Atlanta, GA 30322 USA |
Addresses:
1. Emory Univ, Dept Biol, Atlanta, GA 30322 USA
2. Univ Virginia, Hlth Sci Ctr, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA
3. Baylor Coll Med, Dept Microbiol & Immunol, Houston, TX 77030 USA |
| Publisher: AMER SOC MICROBIOLOGY, 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 265DY |
| ISSN: 0270-7306 |
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