| | |  | | | | Record from Web of Science® | | | | | | |
| Human scribble (Vartul) is targeted for ubiquitin-mediated degradation by the high-risk papillomavirus E6 proteins and the E6AP ubiquitin-protein ligase |
|
|
| Author(s): Nakagawa S, Huibregtse JM |
| Source: MOLECULAR AND CELLULAR BIOLOGY Volume: 20 Issue: 21 Pages: 8244-8253 Published: NOV 2000 |
| Times Cited: 188 References: 55 |
| Abstract: The high-risk human papillomavirus (HPV) E6 proteins stimulate the ubiquitination and degradation of p53, dependent on the E6AP ubiquitin-protein ligase, Other proteins have also been shown to be targeted for degradation by E6, including hDlg, the human homolog of the Drosophila melanogaster Discs large (Dlg) tumor suppressor. We show here that the human homolog of the Drosophila Scribble (Vartul) (hScrib) tumor suppressor protein is also targeted for ubiquitination by the E6-E6AP complex in vitro and that expression of E6 induces degradation of hgcrib in vivo. Characterization of the E6AP-E6-hScrib complex indicated that hgcrib binds directly to E6 and that the binding is mediated by the PDZ domains of hScrib and a carboxyl-terminal epitope conserved among the high-risk HPV E6 proteins. Green fluorescent protein-hScrib was localized to the periphery of MDCK cells, where it colocalized with ZO-1, a component of tight junctions. E6 expression resulted in lass of integrity of tight junctions, as measured by ZO-1 localization, and this effect was dependent on the PDZ binding epitope of E6, Thus, the high-risk HPV E6 proteins induce the degradation of the human homologs of two Drosophila PDZ domain-containing tumor suppressor proteins, hDlg and hScrib, both of which are associated with cell junction complexes. The fact that Scrib/Vart and Dig appear to cooperate in a pathway that controls Drosophila epithelial cell growth suggests that the combined targeting of hScrib and hDIg is an important component of the biologic activity of high-risk HPV E6 proteins. |
| Document Type: Article |
| Language: English |
| Reprint Address: Huibregtse, JM (reprint author), Univ Texas, Inst Mol & Cellular Biol, Sect Mol Genet & Microbiol, Austin, TX 78712 USA |
Addresses:
1. Rutgers State Univ, Dept Mol Biol & Biochem, Piscataway, NJ 08855 USA |
| Publisher: AMER SOC MICROBIOLOGY, 1752 N ST NW, WASHINGTON, DC 20036-2904 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 362TK |
| ISSN: 0270-7306 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |