| | |  | | | | Record from Web of Science® | | | | | | |
| Strand-specific postreplicative processing of mammalian telomeres |
|
|
| Author(s): Bailey SM, Cornforth MN, Kurimasa A, Chen DJ, Goodwin EH |
| Source: SCIENCE Volume: 293 Issue: 5539 Pages: 2462-2465 Published: SEP 28 2001 |
| Times Cited: 130 References: 20 |
| Abstract: Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated endcapping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postrepticative processing of telomeres that is linked to their mode of replication. |
| Document Type: Article |
| Language: English |
| Reprint Address: Goodwin, EH (reprint author), Los Alamos Natl Lab, Biosci Div, POB 1663, Los Alamos, NM 87544 USA |
Addresses:
1. Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87544 USA
2. Univ Texas, Med Branch, Dept Radiat Oncol, Galveston, TX 77550 USA
3. Univ Calif Berkeley, Lawrence Berkeley Lab, Div Cell & Mol Biol, Berkeley, CA 94720 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 476PD |
| ISSN: 0036-8075 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |