| | |  | | | | Record from Web of Science® | | | | | | |
| Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells |
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| Author(s): Zacharias DA, Violin JD, Newton AC, Tsien RY |
| Source: SCIENCE Volume: 296 Issue: 5569 Pages: 913-916 Published: MAY 3 2002 |
| Times Cited: 689 References: 22 |
| Abstract: Many proteins associated with the plasma membrane are known to partition into submicroscopic sphingolipid- and cholesterol-rich domains called lipid rafts, but the determinants dictating this segregation of proteins in the membrane are poorly understood. We suppressed the tendency of Aequorea fluorescent proteins to dimerize and targeted these variants to the plasma membrane using several different types of lipid anchors. Fluorescence resonance energy transfer measurements in living cells revealed that acyl but not prenyl modifications promote clustering in lipid rafts. Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane. |
| Document Type: Article |
| Language: English |
| Reprint Address: Tsien, RY (reprint author), Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA |
Addresses:
1. Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
2. Univ Calif San Diego, Biomed Sci Grad Program, La Jolla, CA 92093 USA
3. Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 549KF |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |