| | |  | | | | Record from Web of Science® | | | | | | |
| Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli |
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| Author(s): Atkinson MR, Savageau MA, Myers JT, Ninfa AJ |
| Source: CELL Volume: 113 Issue: 5 Pages: 597-607 Published: MAY 30 2003 |
| Times Cited: 194 References: 25 |
| Abstract: Analysis of the system design principles of signaling systems requires model systems where all components and regulatory interactions are known. Components of the Lac and Ntr systems were used to construct genetic circuits that display toggle switch or oscillatory behavior. Both devices contain an "activator module" consisting of a modified glnA promoter with lac operators, driving the expression of the activator, NRI. Since NRI activates the glnA promoter, this creates an autoactivated circuit repressible by Lacl. The oscillator contains a "repressor module" consisting of the NRI-activated glnK promoter driving Lacl expression. This circuitry produced synchronous damped oscillations in turbidostat cultures, with periods much longer than the cell cycle. For the toggle switch, Lacl was provided constitutively; the level of active repressor was controlled by using a lacY mutant and varying the concentration of IPTG. This circuitry provided nearly discontinuous expression of activator. |
| Document Type: Article |
| Language: English |
| Reprint Address: Ninfa, AJ (reprint author), Univ Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109 USA |
Addresses:
1. Univ Michigan, Sch Med, Dept Biol Chem, Ann Arbor, MI 48109 USA
2. Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA
3. Univ Michigan, Sch Med, Mol & Cellular Biol Program, Ann Arbor, MI 48109 USA |
| Publisher: CELL PRESS, 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: 687VL |
| ISSN: 0092-8674 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |