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| Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation |
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| Author(s): Dor Y, Brown J, Martinez OI, Melton DA |
| Source: NATURE Volume: 429 Issue: 6987 Pages: 41-46 Published: MAY 6 2004 |
| Times Cited: 655 References: 38 |
| Abstract: How tissues generate and maintain the correct number of cells is a fundamental problem in biology. In principle, tissue turnover can occur by the differentiation of stem cells, as is well documented for blood, skin and intestine, or by the duplication of existing differentiated cells. Recent work on adult stem cells has highlighted their potential contribution to organ maintenance and repair. However, the extent to which stem cells actually participate in these processes in vivo is not clear. Here we introduce a method for genetic lineage tracing to determine the contribution of stem cells to a tissue of interest. We focus on pancreatic beta-cells, whose postnatal origins remain controversial. Our analysis shows that pre-existing beta-cells, rather than pluripotent stem cells, are the major source of new beta-cells during adult life and after pancreatectomy in mice. These results suggest that terminally differentiated beta-cells retain a significant proliferative capacity in vivo and cast doubt on the idea that adult stem cells have a significant role in beta-cell replenishment. |
| Document Type: Article |
| Language: English |
| Reprint Address: Melton, DA (reprint author), Harvard Univ, Dept Mol & Cellular Biol, 7 Divin Ave, Cambridge, MA 02138 USA |
Addresses:
1. Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
2. Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 818CB |
| ISSN: 0028-0836 |
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