| | |  | | | | Record from Web of Science® | | | | | | |
| Structural basis of mitochondrial tethering by mitofusin complexes |
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| Author(s): Koshiba T, Detmer SA, Kaiser JT, Chen HC, McCaffery JM, Chan DC |
| Source: SCIENCE Volume: 305 Issue: 5685 Pages: 858-862 Published: AUG 6 2004 |
| Times Cited: 121 References: 22 |
| Abstract: Vesicle fusion involves vesicle tethering, docking, and membrane merger. We show that mitofusin, an integral mitochondrial membrane protein, is required on adjacent mitochondria to mediate fusion, which indicates that mitofusin complexes act in trans ( that is, between adjacent mitochondria). A heptad repeat region (HR2) mediates mitofusin oligomerization by assembling a dimeric, antiparallel coiled coil. The transmembrane segments are located at opposite ends of the 95 angstrom coiled coil and provide a mechanism for organelle tethering. Consistent with this proposal, truncated mitofusin, in an HR2-dependent manner, causes mitochondria to become apposed with a uniform gap. Our results suggest that HR2 functions as a mitochondrial tether before fusion. |
| Document Type: Article |
| Language: English |
| Reprint Address: Chan, DC (reprint author), CALTECH, Div Biol, 1200 E Calif Blvd,MC114-96, Pasadena, CA 91125 USA |
Addresses:
1. CALTECH, Div Biol, Pasadena, CA 91125 USA
2. CALTECH, Div Chem, Pasadena, CA 91125 USA
3. Johns Hopkins Univ, Howard Hughes Med Inst, Baltimore, MD 21218 USA
4. Johns Hopkins Univ, Dept Biol, Integrated Imaging Ctr, Baltimore, MD 21218 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 843RT |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |