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| Proteome survey reveals modularity of the yeast cell machinery |
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| Author(s): Gavin AC, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Dumpelfeld B, Edelmann A, Heurtier MA, Hoffman V, Hoefert C, Klein K, Hudak M, Michon AM, Schelder M, Schirle M, Remor M, Rudi T, Hooper S, Bauer A, Bouwmeester T, Casari G, Drewes G, Neubauer G, Rick JM, Kuster B, Bork P, Russell RB, Superti-Furga G |
| Source: NATURE Volume: 440 Issue: 7084 Pages: 631-636 Published: MAR 30 2006 |
| Times Cited: 589 References: 43 |
| Abstract: Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set enabled a de novo characterization of the composition and organization of the cellular machinery. The ensemble of cellular proteins partitions into 491 complexes, of which 257 are novel, that differentially combine with additional attachment proteins or protein modules to enable a diversification of potential functions. Support for this modular organization of the proteome comes from integration with available data on expression, localization, function, evolutionary conservation, protein structure and binary interactions. This study provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling. |
| Document Type: Article |
| Language: English |
| Reprint Address: Russell, RB (reprint author), Cellzome AG, Meyerhofstr 1, D-69117 Heidelberg, Germany |
Addresses:
1. Cellzome AG, D-69117 Heidelberg, Germany
2. European Mol Biol Lab, D-69117 Heidelberg, Germany
3. MPI IB, MPI MG, D-10117 Berlin, Germany
4. Austrian Acad Sci, Ctr Mol Med, A-1090 Vienna, Austria |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 026OY |
| ISSN: 0028-0836 |
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