| | |  | | | | Record from Web of Science® | | | | | | |
| New cell lines from mouse epiblast share defining features with human embryonic stem cells |
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| Author(s): Tesar PJ (Tesar, Paul J.), Chenoweth JG (Chenoweth, Josh G.), Brook FA (Brook, Frances A.), Davies TJ (Davies, Timothy J.), Evans EP (Evans, Edward P.), Mack DL (Mack, David L.), Gardner RL (Gardner, Richard L.), McKay RDG (McKay, Ronald D. G.) |
| Source: NATURE Volume: 448 Issue: 7150 Pages: 196-U10 Published: JUL 12 2007 |
| Times Cited: 130 References: 38 |
| Abstract: The application of human embryonic stem (ES) cells in medicine and biology has an inherent reliance on understanding the starting cell population. Human ES cells differ from mouse ES cells and the specific embryonic origin of both cell types is unclear. Previous work suggested that mouse ES cells could only be obtained from the embryo before implantation in the uterus(1-5). Here we show that cell lines can be derived from the epiblast, a tissue of the post-implantation embryo that generates the embryo proper. These cells, which we refer to as EpiSCs (post-implantation epiblast-derived stem cells), express transcription factors known to regulate pluripotency, maintain their genomic integrity, and robustly differentiate into the major somatic cell types as well as primordial germ cells. The EpiSC lines are distinct from mouse ES cells in their epigenetic state and the signals controlling their differentiation. Furthermore, EpiSC and human ES cells share patterns of gene expression and signalling responses that normally function in the epiblast. These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development. |
| Document Type: Article |
| Language: English |
| Reprint Address: McKay, RDG (reprint author), NINDS, Mol Biol Lab, NIH, Bethesda, MD 20892 USA |
Addresses:
1. NINDS, Mol Biol Lab, NIH, Bethesda, MD 20892 USA
2. Univ Oxford, Dept Zool, Mammalian Dev Lab, Oxford OX1 3PS, England
3. NCI, Stem Cell Biol Sect, NIH, Bethesda, MD 20892 USA |
| Publisher: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: 188QN |
| ISSN: 0028-0836 |
| DOI: 10.1038/nature05972 |
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