| AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE |
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| Author(s): ENG JK, MCCORMACK AL, YATES JR |
| Source: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY Volume: 5 Issue: 11 Pages: 976-989 Published: NOV 1994 |
| Times Cited: 2,268 References: 40 |
| Abstract: A method to correlate the uninterpreted tandem mass spectra of peptides produced under low energy (10-50 eV) collision conditions with amino acid sequences in the Genpept database has been developed. In this method the protein database is searched to identify linear amino acid sequences within a mass tolerance of +/- 1u of the precursor ion molecular weight. A cross-correlation function is then used to provide a measurement of similarity between the mass-to-charge ratios for the fragment ions predicted from amino acid sequences obtained from the database and the fragment ions observed in the tandem mass spectrum. In general, a difference greater than 0.1 between the normalized cross-correlation functions of the first- and second-ranked search results indicates a successful match between sequence and spectrum. Searches of species-specific protein databases with tandem mass spectra acquired from peptides obtained from the enzymatically digested total proteins of E. coli and S. cerevisiae cells allowed matching of the spectra to amino acid sequences within proteins of these organisms. The approach described in this manuscript provides a convenient method to interpret tandem mass spectra with known sequences in a protein database. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV WASHINGTON, DEPT MOLEC BIOTECHNOL, SEATTLE, WA 98185 USA |
| Publisher: ELSEVIER SCIENCE INC, 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 |
| Subject Category: Chemistry, Analytical; Chemistry, Physical; Spectroscopy |
| IDS Number: PP713 |
| ISSN: 1044-0305 |